1 materials and methods
1.1 Test materials
Aegis microbial Lysozyme provide by Zhejiang Aegis Biotech Co., Ltd
1.2 Test method
1.2.1 Testing designation
Selected 300 healthy Hyline layers which are 34 week old randomly, using single factor randomized design, divided into five groups, each group have three repeate members, 20 birds per replicate. As the sheet 1 show. Pre-experiment 7d, the test period 56d.
Treat the treatment groups with the same basal diet, Add Lysozyme 5mg/kg, 10mg/kg, 15mg/kg, 20mg/kg to I-IV groups respectively. Regard the V group as the control group.
1.2.2 Feeding management
Cage culture, feed three times daily, appropriate plus amount approved by that there is only bottom material in the next add time, free drinking water, Statistics the feed intake of each group.
1.2.3 Testing of the main raw material of basal diet & the nutrition level
The test basal diet is Liaohe brand formula feed during the laying period which is produced by ABNA (Liaoning) Co., Ltd. ( Feeding period: laying period), show as sheet 2.
1.2.4 drug adding method
Weight different dosage of lysozyme powder respectively, mixed with little basic ration as premix, and then amplificated gradually , mixing in the standby basal ration.
1.2.5 Observate index and testing method
Record the clinical observation, the mental state, diet and defecation of birds after 7 days feeding.
1.2.5.1 Testing of egg laying performance
Records of the eggs number & weight of treatment groups daily, and statistical data of remaining amount of ration in 20th, 30th, 40th, 49th, 10th day. Calculating the egg weight, average egg weight, egg laying rate, feed/egg ratio and feed consumption. The laying rate %=[ Number of eggs / ( hens number * feeding days)] x 100%.
1.2.5.2 collection of egg samples
Randomly taken two eggs from each replicate group which are lay in10th, 20th, 30th, 40th, 49th day respectively, and mark the date & group these eggs, storage them at 4 ℃ in refrigerator and waiting test.
1.2.6 Data analysis
Using SPSS13.0 system analysis software, analyze the test data and Multiple compared with Duncan's method (Duncan).
2 Testing Results
2.1 Clinical Observation
During and after the testing period, the clinical observation of chicken none abnormal, good appetite, normal drinking, good mental condition, normal stool, feathers full of luster, no chicken died during the test.
2.2 Lysozyme effect on production performance of layer
Sheet 3 results showed that: compared with the control group, the II-IV groups' average egg weight are obvious increased (P<0.05); The III-IV groups' egg laying radio are obvious increased (P<0.05); The III-IV groups' feed/egg radio are obvious reduced (P<0.05);
Sheet 3 results showed that: compared with the control group, the II-IV groups' average egg weight are obvious increased (P<0.05); The III-IV groups' egg laying radio are obvious increased (P<0.05); The III-IV groups' feed/egg radio are obvious reduced (P<0.05);
3 Discussion
3.1 Effect on Laying hens’ health of Lysozyme
Lysozyme is a non-toxic, harmless natural protease.There is no any effect on the cells of animals. It is a very safe and nutritious feed additive. Lysozyme can not only change the intestinal microbial colonies, promote the digestion and absorption of food, improve the utilization rate of feed, but also have strong thermal stability, acid resistance, easy to storage and transportation. Lysozyme is a kind of high security additives. According to the clinical observation, in the whole experimental period, the experimental group has no abnormal situation, the whole groups are healthy. The experiment showed that the addition of lysozyme had no adverse effect on the growth and health of laying hens.
3.2 Effects on egg production of Lysozyme
The broilers feed by Shao Chunrong etc proved that, lysozyme can be used to control gastroenteritis, indigestion and suppurative processes of livestock and poultry, what's more, it could also work similar as non - specific growth hormone; Li Xiaohui etc. studied and found that, lysozyme has a close contact with immunoglobulin on function, and could also complement with other biological active substances to enhance its antibody activity , in order to kill bacteria.
Qiu Weihai supplemented with 500mg lysozyme in piglets ration, diarrhea rate decreased by 5% ~ 50% compared to the control group, controlled the piglet diarrhea basically, and that lysozyme as natural bifidus growth factor, could promote the growth of bifidobacterium, supplement suitable quantity lysozyme in ration of young animal could make ration to be emulsion, in order to promote the formation and stability of intestinal micro flora. Zhang Shiqing et al reported after supplement suitable quantity lysozyme in feed, the energy, nutrients metabolic rate and immune index of broilers were increased. This experiment also proves that lysozyme has a certain effect on improving the production performance of layer hens.
3.3 Effects on egg quality of Lysozyme
Test results show that after supplement of lysozyme in the feed, the index of eggs eggshell thickness, eggshell strength, specific gravity, broken&softies rate, yolk colorimetric and Ha s unit have improved, but the difference was not significant. It has been shown before that the lysozyme could improve the egg weight, egg laying rate and feed/egg rate, and ensure the healthy production of laying hens, reduce the disease occurrence, good health effects, improve the egg production performance to some extent.
4 Conclusion
① There was no adverse reaction of lysozyme in egg laying hens;
② Supplement of Lysozyme in ration could improve the production performance of laying hens, the best additive amount is 15mg/kg;
③Lysozyme can improve the laying performance of hens in a certain extent.
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Email: crystal@aegisgroup.cn
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