Effects on growth performance and immune function of Broilers
From: FEED RESEARCH NO. 8, 2009
Released by Lu Yaping, Chen Zhanghong, Pan Hongtao
- Hangzhou Kangyuan Feed Science Co., Ltd
- Zhejiang Tianke High-Tech Dov. Co., Ltd
Researcher: Lu Yaping, Chen Zhanghong, Pan Hongtao
Probiotics can improve the growth performance and immune function of animals by establishing the dominant bacteria group, promoting the nutrients absorption and producing antibacterial substances. Immune polysaccharide could promote the growth of beneficial bacteria, inhibit the growth of pathogenic bacteria, improve the growth performance of animals mainly by improve animal digestive tract flora.
As a non-specific immune factor, lysozyme is involved in many kinds of immune responses, improving and enhancing the phagocytosis and digestion of macrophages, enhancing the body's resistance to maintain the balance of intestinal flora and promoting the growth of animals.
This test mainly study the effects on growth performance and immune function in broilers of lysozyme, probiotics and Astragalus Polysaccharide.
1 materials and methods
1.1 Test materials
Lysozyme was provided by Zhejiang Aegis Biotech Co., Ltd; Lactobacillus and Bacillus were provided by Xi'an Canghai Biological Co., Ltd; Aastragalan was provided by Sichuan Deyang city Hoycome Bio-Tech Co., Ltd.
- 2 Testing animal & designation
Select 288 1 day old healthy Avian broilers, divided into six groups randomly, each group have four repeat, 12 chickens in each replicate. Feed them ration, 100g/t lysozyme supplement ration, 5 billion CFU/g probiotics supplement ration and 320 g/t astragalan supplement ration.
The test ration were formulated with corn and soybean meal, and the nutrition level was shown in Sheet 1.
- 3Measurement index
- 3. 1 Determination of growth performance
Weighting the 1st, 21st and 42nd days broiler, calculate the feed consumption, the daily average weight gain, average daily consumption and feed conversion rate of each stage.
- 3. 2 Determination of serum biochemical parameters
In the first day of the 3rd and 6th week morning, selected 3 broilers from each replicate randomly, prepared heart blood, make serum samples, test the contect of Lysozyme and the activity of total protein (TP), total cholesterol (TC), glucose (Glu) and aspartate transaminase (GPT), glutamic pyruvic transaminase (GPT). Serum biochemical indicators were determined according to the kit instructions.
1.4 statistical method
The test data were analyzed by SPSS10. 0. Variance analysised and LSD multiple comparised by One-Way, and mark the result by Average±Standard error, and multiple comparison.
2 Results and Analysis
2.1 Effect of lysozyme, probiotics and Astragalus Polysaccharide on growth performance of Broilers
Sheet 2 shows that: compared with the control group, supplement lysozyme, probiotics and astragalan into ration can improve broiler growth performance and feed conversion rate. Among them, compared to the control group, lysozyme increased 7.79%, the difference was significant, the Feed reward increase 3.93%, the difference was significant. Compared to astragalan, the weight of Lysozyme group increased 2.43%, the feed reward increased 2.86%. Compared with the control group, the weight of probiotic group increased of 6.55%, the difference was significant; Compared with the control group, the weight of astragalan increased of 5.25%, the difference was significant.
2.2 Effect of lysozyme, probiotics and Astragalus Polysaccharide on the immune function of Broilers
Sheet 2 shows that: Supplement lysozyme into the early growth broiler's ration could improve the content of lysozyme in serum of broilers, compared to the control group, the content of lysozyme in serum increased 43. 16%, difference is very significant; Compared to the probiotic and and astragalan group,increased 34. 36% and 22. 84%, the difference was significant. Lysozyme could increase the total protein content in serum, compared with the control group, increased 41.58%, the difference was significant. Probiotics and astragalan can reduce the content of serum glucose and alanine aminotransferase effectively, compared with the control group, reduced 10. 93%,10. 98% and 38. 71%, 35. 75% respectively, the difference was significant.
Sheet 4 shows that: compared with the other groups, supplement lysozyme into the ration can improve the serum lysozyme content of broilers, increased by 33. 3% (P < 0. 05), 21. 5% (P < 0. 05) and 15. 8% (P > 0. 05); Reduce the content of cholesterol in serum, reduced by 11. 4% (P < 0. 05) and 8% (P < 0. 05) and 7. 9% (P< 0.05). Supplemente with lysozyme, probiotics and astragalus polysaccharide could increased the content of total protein, reduce the content of serum glucose and glutamic pyruvic transaminase in serum.
3 Conclusion
Supplement lysozyme and probioticsy etc into the ration of young broiler could improve the growth performance, especially inprove the early growth performance; they also have different degrees impact on blood biochemical indexes. The weight gain and feed rward of lysozyme group were higher than in the other experimental groups, differences are significant; At the same time, lysozyme can improve broiler Serum Lysozyme and total protein content significantly, improve the immune function.
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